• We study how proteins work by watching them move.

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• Using time-resolved X-ray crystallography at XFELs and synchrotrons, we capture proteins during function.

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• These experiments generate molecular movies that reveal enzyme dynamics in real time.

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• Our research centers on flavoenzymes, redox-active proteins, and antibiotic-resistance enzymes, where small changes have large effects.

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• By combining room-temperature structures and computational modeling, we link structure, dynamics, and function.